Background

In this task, you will complete the first two reactions in the published figure. These include the conversion from glucose to glucose-6-phosphate, and the interaction from glucose-6-phosphate to the Pentose Phosphate Pathway.

Your Mission

Draw the conversion reactions highlighted in the below figure.

  • Launch PathVisio and start a new pathway under File > New.
  • Add metabolite nodes for glucose and glucose-6-phosphate.
  • To add the pathway node, select PATH from the Data Node palette in the Objects tab.
  • Click on the canvas to place the node.
  • Double-click on the node to open its properties.
  • Instead of searching for an ID, enter the id “WP134” in the Identifier field, and enter “WikiPathways” in the Database field.
  • Type in “Pentose Phosphate Pathway” as the Text label. Click OK.
  • A pathway node is by default of Shape Type "None". Update the Shape Type to "RoundedRectangle" for the “Pentose Phosphate Pathway” node.
  • Draw the mim-conversion arrows between the glucose and glucose-6-phosphate nodes, and add an anchor to it.
  • For the interaction between glucose-6-phosphate node and the pentose phosphate pathway node, add the default arrow interaction.
  • Add the gene products that catalyze the conversion between glucose and glucose-6-phosphate, HK1. For the interaction between glucose-6-phosphate and the pentose phosphate pathway, we will not add the catalyzing enzyme node (G6PD), since this interaction is generic and does not end with a specific metabolite.
  • Add the two mim-catalysis arrows for HK1.
  • To add the glycolysis label, select Label from the Graphical elements palette in the Objects tab.
  • Double-click the label to open its properties.
  • Type in “Glycolysis“ and click OK.
  • With the label still selected, in the Properties tab, check the Bold checkbox to make the label bold.
  • Done!
  • Save your work as a GPML file under File > Save As.
  • Drag-and-drop the GPML file below to check if it is correct.